Publications by authors named "R Luongo"
The generation of human pluripotent stem cell (hPSC)-derived brain organoids is continuously refined, enhancing their reproducibility and complexity. Here, we present a guided differentiation protocol for generating cortical forebrain organoids and cortico-pericyte (CP)assembloids composed of a robust outer radial glia (oRG) population and an expanded outer subventricular zone (oSVZ). We describe the steps to generate hPSC-derived cortical organoids (COs), cortical pericytes, and CP assembloids.
View Article and Find Full Text PDFOuter radial glia (oRG) emerge as cortical progenitor cells that support the development of an enlarged outer subventricular zone (oSVZ) and the expansion of the neocortex. The in vitro generation of oRG is essential to investigate the underlying mechanisms of human neocortical development and expansion. By activating the STAT3 signaling pathway using leukemia inhibitory factor (LIF), which is not expressed in guided cortical organoids, we define a cortical organoid differentiation method from human pluripotent stem cells (hPSCs) that recapitulates the expansion of a progenitor pool into the oSVZ.
View Article and Find Full Text PDFPurpose: To investigate and analyze the effects of different prosthetic protocols under different loading and occlusal conditions on the survival rates of single implants immediately placed into fresh extraction sockets of maxillary or mandibular premolars with single-stage surgery.
Materials And Methods: Patients needing replacement of a single premolar in the maxilla or mandible were included and randomly divided into three groups based on the different loading protocols: group 1 = healing abutment; group 2 = provisional crown left out of occlusion without functional loading; and group 3 = provisional crown in functional occlusion in maximum intercuspation without contact in excursions. The hypothesis was that single implants inserted into fresh extraction sockets and immediately connected to a temporary crown under functional loading would demonstrate survival rates comparable to single implants placed in the same conditions connected to a healing abutment or to an immediate temporary crown left out of occlusion.
Maxillary Premolars with Four Canals: Case Series.
Bioengineering (Basel)
December 2022
Article Synopsis
- The case series explores the complexities of maxillary premolars that have four canals, aiming to improve knowledge and treatment strategies.
- It details the endodontic treatment of five specific patients' premolars, highlighting various approaches used in the process.
- Key techniques include careful canal preparation with hybrid shaping, microsurgery, and biomaterial application, while emphasizing the importance of using a dental microscope and thorough radiographic evaluation.
Article Synopsis
- The study aimed to compare two methods for reconstructing post-extraction bone defects: using a non-resorbable d-PTFE membrane left exposed versus guided bone regeneration (GBR) performed later, over a period of four weeks and two months, respectively.
- Conducted as a multicenter randomized controlled trial in Italy with 18 enrolled patients, the study found no instances of implant failure or complications, and all patients reported satisfaction with their implants.
- While histological analyses showed well-mineralized bone in both groups, the regenerated bone appeared more mature in the GBR group, though overall there were no significant differences in tissue volume between the two methods.