SARS-CoV-2 human challenge reveals biomarkers that discriminate early and late phases of respiratory viral infections.

Blood transcriptional biomarkers of acute viral infections typically reflect type 1 interferon (IFN) signalling, but it is not known whether there are biological differences in their regulation that can be leveraged for distinct translational applications. We use high frequency sampling in the SARS-CoV-2 human challenge model to show induction of IFN-stimulated gene (ISG) expression with different temporal and cellular profiles. MX1 gene expression correlates with a rapid and transient wave of ISG expression across all cell types, which may precede PCR detection of replicative infection.

Recovery of purple non-sulfur bacteria-mediated single-cell protein from domestic wastewater in two-stage treatment using high rate digester and raceway pond.

Wastewater resources can be used to produce microbial protein for animal feed or organic fertiliser, conserving food chain resources. This investigation hasemployed thefermented sewage to photoheterotrophically grown purple non-sulfur bacteria (PNSB) in a 2.5 m pilot-scaleraceway-pond with infrared light to produce proteinaceous biomass.

Quantifying genome-wide transcription factor binding affinities for chromatin using BANC-seq.

Transcription factors (TFs) bind specific DNA sequences to regulate transcription. Apart from DNA sequences, local factors such as DNA accessibility and chromatin structure determine the affinity of a TF for any given locus. Including these factors when measuring TF-DNA affinities has proven difficult.

High nitrite accumulation in hydrogenotrophic denitrification at low temperature: Transcriptional regulation and microbial community succession.

High Pressure Hydrogenotrophic Denitrification (HPHD) provided a promising alternative for efficient and clean nitrate removal. In particular, the denitrification rates at low temperature could be compensated by elevated H partial pressure. However, nitrite reduction was strongly inhibited while nitrate reduction was barely affected at low temperature.