Antigen capture ELISA using a monoclonal antibody for the detection of Mycoplasma bovis in milk.

An enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody (mAb) was developed to detect Mycoplasma (M.) bovis in milk samples from cattle. With this procedure, 1 x 10(5) colony forming units per ml (cfu/ml) milk were routinely detectable.

Preparation and characterization of monoclonal antibodies against Mycoplasma bovis.

Monoclonal antibodies (mabs) against Mycoplasma (M.) bovis were prepared for use in diagnosis of bovine mastitis. From the original 32 hybridomas actively secreting mabs against M.

[Experimental infections of bulls with Mycoplasma (M.) bovis and M. bovigenitalium].

M. bovis or M. bovigenitalium species experimentally used in intrapreputial infection were re-isolated from 7 of 8 bulls.