Decarboxylation of [1-(13)C]leucine by hydroxyl radicals.

The decarboxylation of [1-13C]leucine by hydroxyl radicals was studied by using gas chromatography-isotope ratio mass spectrometry (GC-IRMS) to follow the production of 13CO2. A Fenton reaction between a (Fe2+)-porphyrin and hydrogen peroxide under aerobic conditions yielded hydroxyl radicals. The decarboxylation rates (VLeu) measured by GC-IRMS were dependent on [1-13C]leucine, porphyrin and hydrogen peroxide concentrations.

Oxidative decarboxylation of benzoic acid by peroxyl radicals.

A chemical model based on the thermal decomposition of AAPH (2,2'-azobis(2-amidinopropane) dihydrochloride is used for the production of peroxyl radicals. Peroxyl radicals induces the decarboxylation of [7-13C]benzoic acid and the production of 13CO2, which is measured by gas chromatography-isotope ratio mass spectrometry (GC-IRMS). The decarboxylation depends on temperature, AAPH, and benzoic acid concentrations.

[Cardiac arrest and vinorelbine? Apropos of a case and review of the literature].

Cardiotoxicity is not a well-known complication of the administration of vinorelbine (Navelbine). We report a case of cardiogenic shock with pulmonary edema after administration of vinorelbine within an association of radiotherapy and chemotherapy.

Reverse isotope dilution analysis of 13CO2 using gas chromatography/isotope ratio mass spectrometry: application to the quantitative determination of 13CO2 released by human polymorphonuclear leukocytes.

We propose a new method for the quantitative determination of carbon dioxide released by a biological microgenerator: a suspension of human polymorphonuclear leukocytes (PMNL). This method is based on the reverse isotope dilution analysis by gas chromatography/isotope ratio mass spectrometry of 13CO2 released by PMNL in a controlled isotope abundance atmosphere containing 3% CO2. 13CO2 release is effective after PMNL stimulation in the presence of [13C]glucose, labeled on positions 1, 2 or 6.

Evaluation of hydroxyl radicals production using 13CO2 gas chromatography-isotope ratio mass spectrometry.

A gas chromatography-isotope ratio mass spectrometry (GC-IRMS) technique for detecting the production of hydroxyl radicals is described. The decarboxylation of [7-13COOH]benzoic acid in the presence of a hydroxyl radicals source (a mixture of porphyrin and hydrogen peroxide) was followed by direct measurement of the 13CO2/12CO2 isotopic ratio. The production of hydroxyl radicals by the mixtures of porphyrin-hydrogen peroxide was proved by comparative study with electron spin resonance spectrometry and high-performance liquid chromatography analysis of hydroxylation products of benzoic acid.