Publications by authors named "R L von Holtz"
Article Synopsis
- The blockage of miRNA biogenesis by proteins like LIN28 can lead to the activation of oncogenic genes, potentially fueling tumor growth and drug resistance.
- Researchers have created a cell-based assay to find small molecules that boost mature let-7 miRNA levels by inhibiting LIN28B, using a system with a GFP reporter to track this process.
- Screening around 4000 compounds, they identified several that increase miRNA levels and found that Kenpaullone and BIO effectively suppress MYCN protein in neuroblastoma cells, paving the way for new cancer drug screenings and potential therapeutic targets.
Article Synopsis
- The study aimed to evaluate the occurrence of injuries to the infrapatellar branch of the saphenous nerve (IPBSN) after ACL reconstruction using an oblique incision for hamstring graft.
- Out of 59 knees evaluated, 27 (45.7%) experienced sensory disorders post-surgery, with 92.6% of these cases persisting for six months, though only one knee had compromised daily activities.
- The results indicated that the oblique incision did not prevent IPBSN injuries, and there were no significant differences in sensitivity issues based on age, side affected, incision angle, or distances measured, though larger incisions were noted in those without sensitivity changes.
Examining the genomic influence of topically applied probiotics in vitro.
Int J Cosmet Sci
December 2024
Objective: Recent work examined the influence of topically applied just-add-water creams containing Lactobacillus plantarum probiotic cultures on to reconstructed human epidermis (RHE). The ability to blend various quiescent probiotic powders in topical systems allows for the examination of these powders on RHEs employing various individual quiescent probiotics using human gene microarrays.
Methods: Four topical Just-Add-Water powders (STRATABIOSYS™ Technologies) were prepared containing the following: (1) Lactobacillus plantarum Lp90 [200 M CFU/g]; (2) Saccharomyces cerevisiae [200 M CFU/g]; (3) Streptococcus thermophilus [200 M CFU/g]; and (4) Lactococcus Lactis LLa61 [200 M CFU/g].
Introduction: Delivering living probiotics to the skin can be challenging as most water-containing cosmetic products require preservatives to maintain product stability. A recently introduced powdered technology [Stratabiosys™, Vantage Personal Care] allows for quiescent probiotic powders to be stored for extended periods of time. The powders can then be reconstituted to creams at the point of use by adding water and mixing and were examined in vitro on reconstructed human full thickness tissues to see if the probiotic had any influence of several important biomolecules expressed in the skin.
View Article and Find Full Text PDFPyrroloquinoline Quinone Disodium (PQQ2Na) Has an NLRP Inflammasome-Induced Caspase-1 Release Influence in UVB-Irradiated but Not ATP-Treated Human Keratinocytes but Has No Influence in Increasing Skin Cell Mitochondrial Biogenesis in Either Human Keratinocytes or Fibroblasts.
Clin Cosmet Investig Dermatol
January 2022
Introduction: Pyrroloquinoline quinone is a bacterial-derived redox factor that has been shown to have numerous benefits in humans. Recently, a model for examining the ability of normal human epidermal keratinocytes (NHEKs) to demonstrate anti-inflammatory benefits via nod-like receptor protein (NLRP)-activated caspase-1 release was reported. The question of whether PQQ2Na might have anti-inflammatory benefits that function through NLRP-activated release of active caspase-1 has not been explored.
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