The prone-oblique surgical position.

The prone-oblique surgical position as introduced and used by Dr. Malis at Mount Sinai Hospital is presented. The advantages of this position over the prone and knee-chest positions are noted.

Operative microphotography: a Malis legacy.

An account of the methods and equipment used by Dr. Malis for the development of operative microphotography is presented. Attention to the critical evaluation of technology became part of the legacy provided to those he trained.

Anterior-approach cervical diskectomy under the operating microscope.

Since Cloward's description of it in 1958, the anterior approach to the cervical spine for excision of degenerated cervical disk causing radiculopathy and myelopathy and for excision of secondary bony spurs has become relatively standard for most spine surgeons. The procedure is easy to perform in trained hands, is a low-risk surgical operation, and has gratifying results provided patients are well selected. Many surgeons use an operating microscope while doing the procedure.

Growth of immortal simian virus 40 tsA-transformed human fibroblasts is temperature dependent.

Simian virus 40 (SV40)-mediated transformation of human fibroblasts offers an experimental system for studying both carcinogenesis and cellular aging, since such transformants show the typical features of altered cellular growth but still have a limited life span in culture and undergo senescence. We have previously demonstrated (D. S.

Restriction of human adenovirus replication in Chinese hamster cell lines and their hybrids with human cells.

We have found that the replication of human adenovirus (Ad2) is restricted in multiple Chinese hamster cell lines including CHO and V79. The major site of restriction involves differential accumulation of late viral proteins as demonstrated by immunofluorescence assay and polyacrylamide gel electrophoresis with and without prior immunoprecipitation. Synthesis of fiber and penton base are markedly reduced, whereas others, such as the 100K polypeptide, are synthesized efficiently.