Publications by authors named "R L Pemith R Liyanage"
Article Synopsis
- The polysaccharide capsule of pneumococcus protects the bacteria from the host immune system and is the main target for existing vaccines; however, the effectiveness of these vaccines can be compromised by variations in the capsule structure.
- Recent research has identified a new capsule type, 20C, which differs from the previously known B subtype due to gene inactivation affecting the capsule structure.
- There is a need for advanced genetic screening and bioinformatics to monitor mutations in capsule-related genes, which could influence vaccine effectiveness and the emergence of new capsule variants.
This study investigates the impact of substituting lactose with maltodextrin in milk-tea formulations to enhance their physicochemical and structural properties. Various lactose-to-maltodextrin ratios (100:0, 90:10, 85:15, 80:20, 75:25) were evaluated in both post-pasteurized and concentrated skim milk-tea (SM-T) and whole milk-tea (WM-T) formulations. Concentration significantly improved the zeta potential, pH, and browning index in both SM-T and WM-T compared to pasteurization.
View Article and Find Full Text PDFThe rapid increase in the production of powdered milk-tea blends is driven by a growing awareness of the presence of highly nutritious bioactive compounds and consumer demand for convenient beverages. However, the lack of literature on the impact of heat-induced component interactions during processing hinders the production of high-quality milk-tea powders. The production process of milk-tea powder blends includes the key steps of pasteurization, evaporation, and spray drying.
View Article and Find Full Text PDFis the main pathogen of chicken necrotic enteritis (NE) causing huge economic losses in the poultry industry. Although dietary secondary bile acid deoxycholic acid (DCA) reduced chicken NE, the accumulation of conjugated tauro-DCA (TDCA) raised concerns regarding DCA efficacy. In this study, we aimed to deconjugate TDCA by bile salt hydrolase (BSH) to increase DCA efficacy against the NE pathogen .
View Article and Find Full Text PDFArticle Synopsis
- The CRISPR/Cas nucleases system is a key tool in genome editing, but current methods for predicting its effectiveness often overlook important kinetic factors.
- This study introduces a new three-state kinetic mechanism for R-loop formation and identifies critical nucleotides that influence editing accuracy.
- By combining machine learning techniques with energy landscape analysis, the research offers insights that could improve the design and reliability of CRISPR/Cas9 gene editing.