Long-term safety of lentiviral or gammaretroviral gene-modified T cell therapies.

Long-term risks of gene therapy are not fully understood. In this study, we evaluated safety outcomes in 783 patients over more than 2,200 total patient-years of observation from 38 T cell therapy trials. The trials employed integrating gammaretroviral or lentiviral vectors to deliver engineered receptors to target HIV-1 infection or cancer.

Development of a Brief Client Satisfaction and Quality Improvement Tool for Hospital-Based Violence Intervention Programs: Opportunities for Enhancing Client Perspectives.

Hospital-based violence intervention programs (HVIPs) are evidence-informed strategies to promote recovery among victims of violence. Limited tools exist to capture client-reported perspectives of program relevance, responsiveness, acceptability, and impact. We conducted a quality improvement project to develop an HVIP-specific tool that can be used to collect information regarding client satisfaction with services to inform ongoing and future program improvement efforts.

The introduction of the SARS-CoV-2 BA.4 lineage into Pakistan.

Pakistan has experienced a total of six COVID-19 waves throughout the pandemic, each driven by distinct SARS-CoV-2 lineages. This study explores the introduction of Omicron lineage BA.4 into Pakistan, which contributed to the sixth wave between June and September 2022.

A Rapid Host Response Blood Test for Bacterial/Viral Infection Discrimination Using a Portable Molecular Diagnostic Platform.

Background: Difficulty discriminating bacterial versus viral etiologies of infection drives unwarranted antibacterial prescriptions and, therefore, antibacterial resistance.

Methods: Utilizing a rapid portable test that measures peripheral blood host gene expression to discriminate bacterial and viral etiologies of infection (the HR-B/V assay on Biomeme's polymerase chain reaction-based Franklin platform), we tested 3 cohorts of subjects with suspected infection: the HR-B/V training cohort, the HR-B/V technical correlation cohort, and a coronavirus disease 2019 cohort.

Results: The Biomeme HR-B/V test showed very good performance at discriminating bacterial and viral infections, with a bacterial model accuracy of 84.

Hydronium Ions Are Less Excluded from Hydrophobic Polymer-Water Interfaces than Hydroxide Ions.

The cloud point temperatures of aqueous poly(-isopropylacrylamide) (PNIPAM) and poly(ethylene) oxide (PEO) solutions were measured from pH 1.0 to pH 13.0 at a constant ionic strength of 100 mM.