Publications by authors named "R L Hare"
Article Synopsis
- The study aimed to standardize qPCR procedures for diagnosing Mucorales by examining factors that influence DNA extraction and PCR amplification efficiency.
- Two panels of samples were distributed to 26 laboratories, and results showed that using larger serum volumes for DNA extraction and larger input volumes for PCR significantly improved detection sensitivity.
- Findings highlight the importance of optimizing laboratory techniques to enhance diagnosis of Mucormycosis, a serious fungal infection, which is critical for timely treatment in vulnerable patients.
A new approach to sample preparation and enzymatic digestion in bottom-up proteomics has been developed using alginate-based hydrogel entrapment of enzymes. This hydrogel facilitates rapid and room-temperature digestions with multienzyme capabilities. Three methodologies were tested: within microcentrifuge tubes, pipette tips, and automated robotic liquid handling.
View Article and Find Full Text PDFCommunity engagement in a seaside town: evaluation of Good Grief Weston festival.
Palliat Care Soc Pract
September 2024
Article Synopsis
- The Good Grief Weston festival, co-designed with the community, aimed to enhance death and grief literacy in Weston-super-Mare, attracting around 3000 attendees over 8 days in May 2023.
- A mixed methods evaluation, including online surveys and focus groups, was conducted to assess participants' experiences and gather insights for future events.
- Results showed that 71.3% of participants felt more confident discussing grief after attending, with many expressing positive feedback about the festival's impact on their understanding and sense of community.
Bacteriophage Curie is a podovirus that infects . The Curie genome spans 16,810 bp, has 90 bp terminal inverted repeats, and includes 23 protein-coding genes. Its genome architecture resembles phage PineapplePizza and other phi29-like phages.
View Article and Find Full Text PDFAzole-resistant Aspergillus fumigatus (ARAf) fungi have been found inconsistently in the environment in Denmark since 2010. During 2018-2020, nationwide surveillance of clinical A. fumigatus fungi reported environmental TR/L98H or TR/Y121F/T289A resistance mutations in 3.
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