Development of a simple and highly sensitive virion concentration method to detect SARS-CoV-2 in saliva.

Background: Controlling novel coronavirus pandemic infection (COVID-19) is a global challenge, and highly sensitive testing is essential for effective control. The saliva is a promising sample for high-sensitivity testing because it is easier to collect than nasopharyngeal swab samples and allows large-volume testing.

Results: We developed a simple SARS-CoV-2 concentration method from saliva samples that can be completed in less than 60 min.

Ixodid ticks from wild and domestic animals in East and Central Asian flyways.

Tick-borne diseases have a significant impact on human and animal populations, posing an increasing threat to public health, particularly in the context of climate change. Along with the various natural hosts of ticks, birds play a notable role in transmitting ticks and tick-borne pathogens, indicating the importance of monitoring flyways and establishing a cooperative network for comprehensive surveillance and to collect diverse tick samples across various regions. This study aimed to develop an international network for surveillance of disease, collection of sufficient tick samples, and overall identification of the geographical distribution of host and ticks in Asian regions, especially in 11 countries on East Asian and Central Asian flyways.

Molecular detection of and in pet dogs in Metro Manila and Laguna, Philippines.

Background And Aim: and are zoonotic vector-borne pathogens that can infect dogs and humans. Data on and in dogs in the Philippines are lacking. This study was conducted to validate previous reports and further investigate the occurrence of and spp.

Molecular Detection and Characterization of and in Cattle and Water Buffaloes from Southern Luzon, Philippines.

and are tick-borne protozoan parasites that can cause significant economic losses in the cattle industry. This study aimed to contribute to the limited epidemiological data on as well as and in large ruminants in the Philippines. Blood samples of 412 cattle and 108 water buffalo collected from four provinces in Southern Luzon, Philippines, were initially tested for the gene of piroplasms through nested PCR.