A piezoelectric method for monitoring formaldehyde induced crosslink formation between poly-lysine and poly-deoxyguanosine.

To date, no experimental technique has been used to monitor DNA-protein crosslink formation in real-time. Real-time data is important for understanding the underlying chemical mechanisms associated with this reaction process. Here, the novel adaptation of existing piezoelectric quartz crystal (PQC) or quartz crystal microbalance (QCM) technology was used to monitor, in real-time, the formation of a crosslink bond induced by formaldehyde between lysine and guanine.

Piezoelectric quartz crystal biosensors.

Biosensing methods utilize the intrinsic selectivity of a biorecognition process to create relatively simple, low cost, analytical alternatives for a variety of research investigations. Here, biosensor applications of the piezoelectric quartz crystal (PQC) are reviewed. The discussion is divided into sections focusing on the development of PQC based analytical techniques, applications in solution phase sensing pertaining to PQC biosensors, and the current state of knowledge in PQC biosensing applications.