Simultaneous profiling of native-state proteomes and transcriptomes of neural cell types using proximity labeling.

Deep molecular phenotyping of cells at transcriptomic and proteomic levels is an essential first step to understanding cellular contributions to development, aging, injury, and disease. Since proteome and transcriptome level abundances only modestly correlate with each other, complementary profiling of both is needed. We report a novel method called simultaneous protein and RNA -omics (SPARO) to capture the cell type-specific transcriptome and proteome simultaneously from both in vitro and in vivo experimental model systems.

Synergistic effects of hydrogen sulfide and nitric oxide in enhancing salt stress tolerance in cucumber seedlings.

Salinity stress poses a significant threat to plant growth and agricultural productivity, affecting millions of hectares of land worldwide. The adverse effects of salt toxicity, primarily caused by high levels of sodium chloride in soil and water, disrupt essential physiological processes in plants, leading to reduced yields and degraded soil quality. The present study thoroughly investigated the potential involvement of hydrogen sulphide (HS) and nitric oxide (NO) in facilitating salt stress tolerance in cucumbers.

Navigating Antibiotic Resistance in Gram-Negative Bacteria: Current Challenges and Emerging Therapeutic Strategies.

The rapid rise of antibiotic resistance poses a severe global health crisis, necessitating new approaches to counter this growing threat. The problem is exacerbated in Gram-negative bacterial pathogens as many antibiotics are unable to enter these cells owing to their unique additional outer membrane barrier. In this review, we discuss the challenges of targeting Gram-negative bacteria, including the complexity of the outer membrane, as well as the presence of efflux pumps and β-lactamases that contribute to resistance.

Short-term safety and efficacy of ripasudil as "add-on therapy" in glaucoma patients on maximum tolerable glaucoma medication.

Purpose: The purpose of this study was to evaluate the additional intraocular pressure (IOP) lowering effect and tolerability of ripasudil in patients with glaucoma inadequately controlled with maximum tolerable medical therapy.

Methods: A prospective, noncomparative, interventional case series. Patients with primary open-angle glaucoma (POAG) but IOP inadequately controlled with maximum medical therapy were enrolled for this.

Sonographic and Doppler Evaluation of Carotid Artery in Hypertensive and Normotensive Individuals.

Background: Hypertension is a significant risk factor for cardiovascular diseases. Evaluating arterial distensibility can be inconsistent, but the resistive index (RI) measured by Doppler ultrasound is effective in assessing vascular resistance. IMT and RI are valuable in atherosclerosis evaluation.