Publications by authors named "R Krisher"
Gene editing technologies have revolutionized the field of livestock breeding, offering unprecedented opportunities to enhance animal welfare, productivity, and sustainability. This paper provides a comprehensive review of recent innovations and applications of gene editing in livestock, exploring the diverse applications of gene editing in livestock breeding, as well as the regulatory and ethical considerations, and the current challenges and prospects of the technology in the industry. Overall, this review underscores the transformative potential of gene editing in livestock breeding and its pivotal role in shaping the future of agriculture and biomedicine.
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- Produced embryos have lower viability than their natural counterparts, leading to concerns about their development and potential.
- Mammalian embryos can adapt metabolically to different culture media, but this adaptability might compromise their viability and implantation capability despite reaching the blastocyst stage.
- The review aims to summarize the progress in embryo culture media for bovine embryos, focusing on key achievements and ongoing challenges to improve developmental outcomes.
Article Synopsis
- Single-cell analysis of bovine blastocyst transcriptome has identified key cell lineages, including the inner cell mass (ICM), trophectoderm (TE), and transitional cells.
- Comparing embryos derived from in vivo (IVV) and two different in vitro (IVC and IVR) protocols revealed delays in cell fate commitment to ICM in IVC and IVR embryos, affecting their development potential.
- Pathway analysis showed that IVC embryos had reduced cellular signaling and transport activities, leading to lower developmental potential, while IVR embryos exhibited increased signaling but issues with ion balance, resulting in compromised development compared to IVV embryos.
Chemically defined oocyte maturation media supplemented with FGF2, LIF, and IGF-1 (FLI medium) enabled significantly improved oocyte quality in multiple farm animals, yet the molecular mechanisms behind such benefits were poorly defined. Here, we first demonstrated that FLI medium enhanced mouse oocyte quality assessed by blastocyst formation after in vitro fertilization and implantation and fetal development after embryo transfer. We then analyzed the glucose concentrations in the spent media; reactive oxygen species concentrations; mitochondrial membrane potential; spindle morphology in oocytes; and the abundance of transcripts of endothelial growth factor-like factors, cumulus expansion factors, and glucose metabolism-related genes in cumulus cells.
View Article and Find Full Text PDFThe refinement of embryo culture media is essential in improving embryo viability and in vitro production efficiency. Our previous work demonstrated that the nutrients (carbohydrates, amino acids, and vitamins) in traditional culture media far exceed the need for an embryo and producing developmentally competent embryos in a reduced nutrient environment is feasible. Here, we aim to evaluate the impact of exogenous lipid and L-carnitine supplementation on bovine blastocyst development and refine our RN condition further.
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