[Contractile activity and sensitivity of smooth muscle cells of the small intestine to agonists in acute ileus].

The impact of acute ileus on the contractility and sensitivity of small intestinal smooth muscle cells to acetylcholine and histamine, as well as possible recovery of smooth muscle cells after removal of 3-hour acute ileus were studied. The contractility of small intestinal smooth muscle cells and their sensitivity to the agonists decreased in proportion to the duration of acute ileus only in the strangulated portion of the bowel. Removal of 3-hour acute ileus resulted in a stepwise recovery of the contractility and sensitivity of small intestinal smooth muscle cells to the agonists in the adducting, strangulated, and abducting portions.

[The ultrastructure of smooth muscle cells under static stretching].

Smooth muscle cells of the guinea pig vas deferens in the organotypic culture change their phenotype from the contractile to the synthetic one within a month. The activating nucleoli, while increasing the number of free ribosomes and endoplasmic reticulum, decreased the amount of myofilaments and dense bodies. Longitudinal static stretching maintains the contraction phenotype in the longitudinal layer muscle but not in the circular layer.

[The phasic contraction of the guinea pig vas deferens in organotypic culture during periodic stretching].

Vas deferens explants from the adult guinea pigs were cultivated under organotypic conditions with repeated stretching. In control explants with no stretching over 9 days the occurring hypersensitivity is similar to the denervated one. Simultaneously phase component contractile response decreased in a 5.

[The hypersensitivity of the guinea pig vas deferens in organotypic culture under static stretching].

The explants of the guinea pig vas deferens acquired the same hypersensitivity as the denervated ones in cultivation under organotypic conditions. The hypersensitivity reached its maximum in 9 days. The explants cultivated with static stretching during 9 and 15 days preserved the contractile properties of freshly isolated stria.

[Carbonic anhydrase--marker of cell type in cell culture of the guinea pig vas deferens].

Polyclonal antibodies (PCAB) to smooth muscle myosin (SMM), monoclonal antibodies (MCAB) to cytokeratin 8 (clon HI, IgGI) and H4 (IgM), as well as PCAB to carbonic anhydrase III were used for identification of the cell types in the vas deferens cell culture of guinea pig. Smooth muscle cells (SMC) are identified by intensive staining of PCAB to SMM. Fibroblast-like cells (FBL) are determined by the presence of the filament finest network, apparently responding to the myosin non-muscular forms, which are present in PCAB to SMM.