Investigation of malaria sporozoites by immunoradiometric assay.

Two-site immunoradiometric assay (IRMA) (Zavala et al., 1982) using monoclonal antibodies to P. falciparum and P.

Field applications of an immunoradiometric assay for the detection of Plasmodium falciparum antigen in a population in a malaria-endemic area in Thailand.

Two surveys were made in people living in a malaria-endemic area in West Thailand in October 1985 (a transmission season) and in May 1986 (a nontransmission season) to detect Plasmodium falciparum antigen using the immunoradiometric assay (IRMA). In the first survey involving 101 people, the IRMA-positive rate was 56.4% and then significantly declined to 16.

Two-site immunoradiometric assay for detection of Plasmodium falciparum antigen in blood using monoclonal and polyclonal antibodies.

Three systems of immunoradiometric assays (IRMAs), a two-site monoclonal antibody sandwich IRMA (MAb-IRMA), two-site polyclonal antibody-monoclonal antibody sandwich IRMA (PAb-MAb-IRMA), and two-site polyclonal antibody sandwich IRMA (PAb-IRMA), were developed to detect low-grade infections with Plasmodium falciparum. The assays showed good correlation with parasitemia when tested against parasites from in vitro cultures (r = 0.996, 0.

Studies on the transcapillary escape rate of fibrinogen and capillary permeability in patients with Plasmodium falciparum malaria.

The transcapillary escape rate and capillary permeability of fibrinogen were studied in 7 patients wih Plasmodium falciparum malaria and 4 control subjects by using 131I-fibrinogen as a tracer. The plasma disappearance curve of 131I-fibrinogen was followed during the first 60 minutes after injection. The mean transcapillary rate of fibrinogen in these patients was found to be significantly higher than that of the control group.

Study on the diagnosis of Gnathostoma infection in cats by radioimmunoassay.

A diagnostic method for gnathostomiasis by radioimmunoassay was carried out. Antibody activity produced in cat serum against Gnathostoma antigen was measured by using solid phase method and 125I-labelled anti cat IgG antibody. The result showed that the antibody activity in cats infected with Gnathostoma spinigerum was significantly higher than those of the control group and the Toxocara infected cats even at the dilution of 1:10,000, except in the Opisthorchis infected group at the dilution higher than 1:400.