Development of a High-Throughput Platform for Quantitation of Histone Modifications on a New QTOF Instrument.

Histone post-translational modifications (PTMs) regulate gene expression patterns through epigenetic mechanisms. The five histone proteins (H1, H2A, H2B, H3, and H4) are extensively modified, with over 75 distinct modification types spanning more than 200 sites. Despite strong advances in mass spectrometry (MS)-based approaches, identification and quantification of modified histone peptides remains challenging because of factors, such as isobaric peptides, pseudo-isobaric PTMs, and low stoichiometry of certain marks.

Modeling carbon dynamics from a heterogeneous watershed in the mid-Atlantic USA: A distributed-calibration and independent verification (DCIV) approach.

The terrestrial ecosystem plays a vital role in regulating regional and global carbon budgets. Ecosystem models are extensively employed to estimate carbon fluxes across different spatial scales. However, there remains a need to reduce the uncertainties associated with model parameterization and input data.

Chungtaejeon (CTJ) inhibits adhesion and migration of VSMC through cytoskeletal remodeling pathway.

Introduction: Vascular remodeling is crucial for the progression of vascular disease such as atherosclerosis. We utilize the experimental model of atherosclerosis to elucidate the activity of Chungtaejeon (CTJ), a Korean fermented tea on adhesion and migration of human aortic vascular smooth muscle cells (HASMC).

Materials And Methods: Various assays such as cell viability, cell adhesion, Western blot, immunofluorescence, were carried out on HASMC to explore pathway associated with cytoskeletal remodeling during the progression of atherosclerosis.

Reflection on Five Months Rural District Hospital Posting of Final Year MBBS Students.

Patan Academy of Health Sciences has been sending its undergraduate medical students to rural postings aligning with the national health system of Nepal to produce competent and empathetic healthcare professionals as a part of its social accountability. One such rural posting is a 20-week long district posting where students are posted at district hospital and district health office. We were final-year students posted at Gulmi district for this purpose in the year 2021/22.