Interlaboratory Measurement of Adeno-Associated Virus: Comparative Quantification of Full and Empty Capsids.

Recombinant adeno-associated virus (AAV) is one of the main viral vector-based gene therapy platforms. AAV is a virus consisting of a ≈25 nm diameter capsid with a ≈4.7 kb cargo capacity.

A retrospective, multicentric, nationwide analysis of the impact of splenectomy on survival of pancreatic cancer patients.

Objective: Splenectomy is regularly performed in total and distal pancreatectomy due to technical reasons, lymph node dissection and radicality of the operation. However, the spleen serves as an important organ for competent immune function, and its removal is associated with an increased incidence of cancer and a worse outcome in some cancer entities (Haematologica 99:392-398, 2014; Dis Colon Rectum 51:213-217, 2008; Dis Esophagus 21:334-339, 2008). The impact of splenectomy in pancreatic cancer is not fully resolved (J Am Coll Surg 188:516-521, 1999; J Surg Oncol 119:784-793, 2019).

Nutation Spectroscopy of a Weakly Interacting Heterobimetallic Spin System.

The use of V and Cu spins to design weakly coupled and dissymmetric spin systems has been examined. Such systems were synthesized using porphyrin-based complexes, with external coordination sites allowing for the formation of dimers via a Pd linker ion. Continuous-wave (CW) Electron Paramagnetic Resonance (EPR) spectroscopy allowed the unequivocal magnetic characterization of the mononuclear precursors VO and Cu.

Keto-enol equilibrium: stable tautomers of -, -, and -hydroquinones in large aromatics.

The synthesis of [6]helicene -quinone starting from the 1,4-dimethoxy-[6]helicene derivative is presented. The demethylation reaction with boron tribromide led to unexpected results. Instead of the expected -hydroquinone, the diketone tautomeric form was isolated.

Fast HPLC-based affinity method to determine capsid titer and full/empty ratio of adeno-associated viral vectors.

Recombinant adeno-associated viruses (rAAVs) are promising gene delivery vectors in the emerging field of gene therapies. To ensure their consistent quality during manufacturing and process development, multiple analytical techniques have been proposed for the characterization and quantification of rAAV capsids. Despite their indisputable capabilities for performing this task, current analytical methods are rather time-consuming, material intensive, complicated, and costly, restricting their suitability for process development in which time and sample throughput are severe constraints.