Detection of genomic variants in BRCA1 and BRCA2 across gastric cancer patients using next generation sequencing.

Objectives: To explore the landscape of BRCA1/2 mutations in gastric cancer patients.

Methods: Next-generation sequencing (NGS), Sanger sequencing, reverse transcription quantitative polymerase chain reaction (RT-qPCR), Immunohistochemistry, The Cancer Genome Atlas (TCGA), gnomAD, and DAVID.

Results: With 95% of bases boasting a phred score surpassing 30 and a minimum coverage depth of 500X, our NGS approach ensures high-quality data acquisition.

COMPLETE HEART BLOCK IN PATIENTS PRESENTING WITH ACUTE ANTERIOR WALL MYOCARDIAL INFARCTION.

Background: Complete heart block (CHB) is a relatively common complication in post-acute myocardial infarction (AMI) patients. Data on the incidence and consequences of CHB in patients with ST-segment elevation myocardial infarction (STEMI) are limited. Besides serving as an indicator of widespread myocardial injury, the development of CHB in STEMI is also associated with a higher risk of sudden cardiac death.

Frequency, Risk Factors, and Methods of Deliberate Self-Harm Observed in Medical Students and Doctors in Pakistan.

Objective: To determine the frequency of deliberate self-harm (DSH), risk factors leading to DSH, and the methods adopted by medical students and young doctors to execute DSH.

Method: A cross-sectional study was conducted on medical college students and young doctors aged 18-26 years. After approval by the parent institute's ethical board, different medical students and doctors from Pakistani medical colleges of both male and female populations were recruited through non-probability sampling.