Demonstration of human alpha-L-fucosidase polymorphism by means of monoclonal antibodies.

Conventional rabbit antibodies and mouse monoclonal antibodies were raised to alpha-L-fucosidase purified from human placenta. Four monoclonal antibodies were studied, of which only one (A) was able to immunoprecipitate the fucosidase activity completely. Two antibodies (B and C) precipitated 65% and one (D) 35% of the activity.

A comparative study of ELISA and other methods for the detection of Brucella antibodies in bovine sera.

Enzyme-linked immunosorbent assay (ELISA), using beta-galactosidase and a fluorigenic substrate, was used for the detection of antibodies to Brucella abortus in bovine sera. Among 677 animals from 9 brucellosis-free herds, none reacted in the ELISA. Among 785 animals from 23 brucellosis-infected herds, 336 were positive in ELISA, 229 in the slow agglutination test (SAT), 185 in the complement fixation test (CFT), and 165 in the Rose-Bengal test (RBT).