Two-dimensional gel electrophoretic resolution of the polypeptides of rat liver mitochondria and the outer membrane.

The proteins of highly purified rat liver mitochondria were resolved by two-dimensional polyacrylamide gel electrophoresis, and detected by staining with either Coomassie blue or silver. Approximately 250 polypeptides were detected with silver staining which is 2- to 3-times that observed with Coomassie blue. Silver staining was especially more effective than Coomassie blue for detecting polypeptides of less than 50 000 daltons.

[Diagnosis of tuberculous pleurisy in cultures of prepared and unprepared pathologic material].

During four years in our laboratory work a small number of bacteriologically confirmed tuberculous pleurises was observed. By different procedure of inoculation of Löwenstein-Jensen medium the greater number of clinical diagnosis was confirmed. To avoid negative influence of the base and the acid on Mycobacterium tuberculosis which could be found in smaller number in pleural punctate, inoculation of the bioptic material was performed directly on medium without prior digestion and decontamination.

[Acinetobacter calcoaceticus as a cause of infection in wounds and burns].

Over the period from January 1985 to July 1986 2038 samples of the patients with open wounds and burns, treated in the Military Medical Academy in Belgrade, were tested. Acinetobacter calcoaceticus was found in 152 samples (7.45%).

[The number of chronic patients contributing to total tuberculosis morbidity in the area of the city of Belgrade].

During four years (from 1984-1987) a group of 94 patients with chronic pulmonary tuberculosis were investigated. Mean age of patients was 51 years-the youngest was 27 and the oldest 78. Mean time of disease was 5.