Iron rescues glucose-mediated photosynthesis repression during lipid accumulation in the green alga Chromochloris zofingiensis.

Energy status and nutrients regulate photosynthetic protein expression. The unicellular green alga Chromochloris zofingiensis switches off photosynthesis in the presence of exogenous glucose (+Glc) in a process that depends on hexokinase (HXK1). Here, we show that this response requires that cells lack sufficient iron (-Fe).

Oligosaccharide Le(a)-Le(x): a cell surface marker for carcinomas derived from embryonic endoderm.

An immunohistochemical evaluation of Le(a)-Le(x) expression by adenocarcinomas of the biliary tree, pancreas, colon and stomach was undertaken as examples of epithelial tumors derived from embryonic endoderm. This complements previous studies showing that Lea-Lex was present on the cell surface of non-small cell lung carcinomas, some non-lung carcinomas, and is a prognostic marker for squamous cell lung carcinomas. All of the tumor specimen evaluated were positive and no expression of Le(a)-Le(x) was detected in derivatives of neural, connective or muscle tissues.

Decrease in Le(x) expression in esophageal adenocarcinomas arising in Barrett's epithelium.

Fifty esophageal adenocarcinomas were investigated for their expression of Le(a), Le(x), and Le(a)-Le(x). Among the 50 adenocarcinomas, 17 cases developed in Barrett's epithelium. Those 17 differed from the other 33 cases by expressing much less Le(x).

Cell-cell interactions influence oligosaccharide modifications on mucins and other large glycoproteins.

Intratumoral phenotypic diversity is well documented with regard to tumor associated carbohydrate antigens (TACA). The factors which control the expression of these cell-surface oligosaccharides on different cells of the same tumor are not understood. We investigated the expression of a panel of mucin associated oligosaccharides in cell lines growing at different surface densities (number of cells per cm2 of growth flask).

Attenuated hypercholesterolemic response to a high-cholesterol diet in subjects heterozygous for the apolipoprotein A-IV-2 allele.

Background: Previous studies have suggested that the variant apolipoprotein (apo) allele apo A-IV-2 may influence the response of the plasma cholesterol concentration to dietary cholesterol.

Methods: We measured plasma lipids and lipoproteins in 11 subjects who were heterozygous for the apo A-IV-2 allele (apo A-IV-1/2 heterozygotes) and a control group of 12 subjects who were homozygous for the common apo A-IV allele (apo A-IV-1/1 homozygotes) in an outpatient dietary-modification study. (Approximately one in seven persons in the United States is a heterozygote.