Activation of prostaglandin EP receptors by lubiprostone in rat and human stomach and colon.

Background And Purpose: Lubiprostone (Amitiza), a possible ClC-2 channel opener derived from prostaglandin E(1) and indicated for the treatment of constipation, increases chloride ion transport and fluid secretion into the intestinal lumen. As lubiprostone may also directly modulate gastrointestinal motility, we investigated its actions and the possible involvement of prostaglandin EP receptor activation on rat and human isolated gastrointestinal preparations.

Experimental Approach: Rat and human isolated preparations were mounted in tissue baths for isometric recording.