Publications by authors named "R J Kohel"
Article Synopsis
- Gossypium hirsutum has a complicated genome structure, making it challenging to sequence, but researchers successfully produced a draft genome using extensive sequencing techniques.
- The assembly covers a significant portion of the genome with 88.5% of scaffolds organized into 26 pseudochromosomes, allowing for comparison with related cotton species.
- Key findings include the high percentage of repeated sequences, evidence of active transposable elements, and potential evolutionary adaptations in genes related to fiber production, suggesting a focus on enhancing cotton fiber traits.
Cotton fiber represents the largest single cell in plants and they serve as models to study cell development. This study investigated the distribution and evolution of fiber Unigenes anchored to recombination hotspots between tetraploid cotton (Gossypium hirsutum) At and Dt subgenomes, and within a parental diploid cotton (Gossypium raimondii) D genome. Comparative analysis of At vs D and Dt vs D showed that 1) the D genome provides many fiber genes after its merger with another parental diploid cotton (Gossypium arboreum) A genome although the D genome itself does not produce any spinnable fiber; 2) similarity of fiber genes is higher between At vs D than between Dt vs D genomic hotspots.
View Article and Find Full Text PDFA quantitative trait locus (QTL) mapping was conducted to better understand the genetic control of plant architecture (PA), yield components (YC), and fiber properties (FP) in the two cultivated tetraploid species of cotton (Gossypium hirsutum L. and G. barbadense L.
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- The cotton genome is complex (AADD; 2n = 52), making research difficult, but the Gossypium arboreum (AA; 2n = 26) genome was sequenced and assembled as a contributor to the A subgenome.
- A substantial clean sequence of 193.6 Gb was generated, allowing for 90.4% of the genome to be organized into 13 pseudochromosomes, with 68.5% consisting of repetitive DNA.
- The study identified 41,330 protein-coding genes and revealed shared whole-genome duplications between G. arboreum and Gossypium raimondii, highlighting the importance of certain genes in cotton disease resistance
Although new and emerging next-generation sequencing (NGS) technologies have reduced sequencing costs significantly, much work remains to implement them for de novo sequencing of complex and highly repetitive genomes such as the tetraploid genome of Upland cotton (Gossypium hirsutum L.). Herein we report the results from implementing a novel, hybrid Sanger/454-based BAC-pool sequencing strategy using minimum tiling path (MTP) BACs from Ctg-3301 and Ctg-465, two large genomic segments in A12 and D12 homoeologous chromosomes (Ctg).
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