Publications by authors named "R J Gruninger"
Anaerobic gut fungi (AGF) were the last phylum to be identified within the rumen microbiome and account for 7-9% of microbial biomass. They produce potent lignocellulases that degrade recalcitrant plant cell walls, and rhizoids that can penetrate the cuticle of plant cells, exposing internal components to other microbiota. Interspecies H transfer between AGF and rumen methanogenic archaea is an essential metabolic process in the rumen that occurs during the reduction of CO to CH by methanogens.
View Article and Find Full Text PDFGenome sequence of isolates from liver abscesses in feedlot cattle.
Microbiol Resour Announc
December 2024
Article Synopsis
- - High-grain diets in cattle can lead to liver infections caused by multiple microorganisms, particularly bacteria.
- - Researchers have isolated specific bacteria from the pus in liver abscesses of affected cattle and sequenced their genomes.
- - This genetic information helps researchers better understand how liver abscesses develop in cattle and the underlying mechanisms involved.
Article Synopsis
- The research presents a new method for analyzing glycosidic linkages in polysaccharides from five types of brown seaweed, focusing on structuring their alcohol-insoluble residues (AIRs).
- This method utilizes GC-MS analysis of partially methylated alditol acetates (PMAAs) to identify variations in fucose linkages across different seaweed species, harvest years, and treatments, revealing significant structural diversity.
- It also improves cellulose quantification by addressing shortcomings in traditional methods, and introduces a pretreatment that helps detect uronic acid linkages in alginates more effectively.
Article Synopsis
- * The study explored GEs from specific rumen bacteria and fungi, revealing they share a similar structural design but show variations, particularly at their active sites, which affects how they function.
- * Findings suggest that horizontal gene transfer (HGT) between different species may have played a role in the diversity of GEs in the rumen, highlighting the evolutionary processes that shape enzyme functions in this microbial community.
Although circular RNAs (circRNAs) play important roles in regulating gene expression, the understanding of circRNAs in livestock animals is scarce due to the significant challenge to characterize them from a biological sample. In this study, we assessed the outcomes of bovine circRNA identification using six enrichment approaches with the combination of ribosomal RNAs removal (); linear RNAs degradation (); linear RNAs and RNAs with structured 3' ends degradation (); ribosomal RNAs coupled with linear RNAs elimination (); ribosomal RNA, linear RNAs and RNAs with poly (A) tailing elimination (); and ribosomal RNA, linear RNAs and RNAs with structured 3' ends elimination (), respectively. RNA-sequencing analysis revealed that different approaches led to varied ratio of uniquely mapped reads, false-positive rate of identifying circRNAs, and the number of circRNAs per million clean reads ( <0.
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