Influence of long-term antigenic stimulation started in young C57BL mice on the development of age-related monoclonal gammapathies.

Long-term antigenic stimulation by multiple antigens (DNP conjugated to human serum albumin (DNP-HSA), ovalbumin and pneumococcal polysaccharide) without adjuvant in young C57BL mice resulted in the development of homogeneous immunoglobulins (H-Ig) during aging in frequencies higher than those in the control group. Our data showed that antigen-specific B-cell clones were at least in part responsible for this increased incidence of age-related monoclonal gammapathies (MG). Using in situ adsorption performed on Wieme's agar plates and in immunoelectrophoresis, antibody activity to one of the immunizing agents (DNP-HSA) could be demonstrated within 10% of the in old mice appearing H-Ig components.

The 5T2 mouse multiple myeloma model: characterization of 5T2 cells within the bone marrow.

The transplantable C57BL/KaLwRij mouse 5T2 multiple myeloma (MM) is a new animal model for studies on MM in man. Histological examination of the 5T2 MM cells revealed their morphological heterogeneity. In this study we investigated whether this heterogeneity reflects subpopulations of 5T2 MM cells with different biological properties.

Influence of treatment with APD-bisphosphonate on the bone lesions in the mouse 5T2 multiple myeloma.

The effects of the treatment of multiple myeloma (MM) with APD-bisphosphonate on bone destruction, the dissemination pattern of the MM, and toxicity for normal and malignant cells were investigated in an animal model, the 5T2 MM. This mouse MM very closely resembles the human disease, including the typical bone lesions. It was demonstrated by radiography, microradiography, and histologic investigation that the treatment of the 5T2 MM with APD-bisphosphonate protected the mice against a loss of bone to a significant extent.

Anti-major histocompatibility complex immunity detected prior to intentional alloimmunization. I. Naturally occurring H-2-specific antibodies in C57BL/KaLwRij (H-2b) mice.

Naturally occurring, H-2-specific, lymphocytotoxic antibodies were detected in 3-10% of young adult and in 10-40% of aged C57BL/KaLwRij (H-2b) mice. The antibodies were of the IgM class and occurred in low titers, but occasionally a high titer was found. The antibodies detected public lymphocyte-membrane antigens controlled by genes identical with, or closely linked to class-I H-2K and H-2D genes.