Publications by authors named "R Iyyappan"
Translational regulation is pivotal during preimplantation development. However, how mRNAs are selected for temporal regulation and their dynamic utilization and fate during this period are still unknown. Using a high-resolution ribosome profiling approach, we analyzed the transcriptome, as well as monosome- and polysome-bound RNAs of mouse oocytes and embryos, defining an unprecedented extent of spatiotemporal translational landscapes during this rapid developmental phase.
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- DNA replication timing in differentiated cells is predetermined, but the establishment of this timing during mammalian development is unclear; new research reveals key insights into this process.
- Using single-cell sequencing, scientists found that late replicating regions are linked with the B compartment and nuclear lamina from the very first cell cycle post-fertilization in both maternal and paternal genomes.
- The study indicates that these late replicating areas are prone to chromosome breaks and fragility, particularly affecting long neuronal genes before the differentiation of somatic and germ cell lineages, highlighting early genome organization after fertilization.
Study Question: Which actively translated maternal transcripts are differentially regulated between clinically relevant in vitro and in vivo maturation (IVM) conditions in mouse oocytes and zygotes?
Summary Answer: Our findings uncovered significant differences in the global transcriptome as well as alterations in the translation of specific transcripts encoding components of energy production, cell cycle regulation, and protein synthesis in oocytes and RNA metabolism in zygotes.
What Is Known Already: Properly regulated translation of stored maternal transcripts is a crucial factor for successful development of oocytes and early embryos, particularly due to the transcriptionally silent phase of meiosis.
Study Design, Size, Duration: This is a basic science study utilizing an ICR mouse model, best suited for studying in vivo maturation.
Article Synopsis
- Mammalian oocyte development relies on precise translation of maternal mRNA during critical phases of meiotic and early embryonic stages when transcription stops.
- The protein cytoplasmic polyadenylation element-binding protein 3 (CPEB3) plays a crucial role by stabilizing specific mRNAs needed for embryonic transcription.
- Without CPEB3, oocytes can undergo meiosis but face early embryonic development issues due to disrupted mRNA stability, leading to protein expression problems and failed embryonic transcription initiation.
Article Synopsis
- A thorough understanding of molecular and cellular processes during peri-implantation is crucial for improving pregnancy outcomes, which is when most pregnancy failures happen.
- Researchers analyzed single-cell transcriptomes of bovine embryos at different peri-implantation stages (days 12, 14, 16, and 18) to identify cell types and gene expression patterns in important embryonic structures.
- The study unveiled a new primitive trophoblast lineage and highlighted similarities and differences in developmental processes between bovines and other mammals, emphasizing the importance of cell communication for healthy early development.