Publications by authors named "R Hornberger"
Article Synopsis
- The study focuses on how pioneer transcription factors like Nr5a2 help initiate gene expression from tightly packed DNA (closed chromatin) during processes like cell reprogramming.
- Researchers used cryo-electron microscopy to visualize how NR5A2 interacts with nucleosomes (DNA-protein complexes), revealing that its unique structural features allow it to disrupt this complex and expose DNA for transcription.
- Mutational analysis indicates that while specific parts of NR5A2 are not essential for binding to DNA, they are crucial for maintaining its connection with nucleosomes and promoting DNA accessibility, highlighting its role in enhancing gene expression.
In this study, a 96-well exposure system for safety assessment of nanomaterials is developed and characterized using an air-liquid interface lung epithelial model. This system is designed for sequential nebulization. Distribution studies verify the reproducible distribution over all 96 wells, with lower insert-to-insert variability compared to non-sequential application.
View Article and Find Full Text PDFThe depletion of fossil resources and the growing demand for plastic waste reduction has put industries and academic researchers under pressure to develop increasingly sustainable packaging solutions that are both functional and circularly designed. In this review, we provide an overview of the fundamentals and recent advances in biobased packaging materials, including new materials and techniques for their modification as well as their end-of-life scenarios. We also discuss the composition and modification of biobased films and multilayer structures, with particular attention to readily available drop-in solutions, as well as coating techniques.
View Article and Find Full Text PDFThe generation of a chemical system capable of replication and evolution is a key objective of synthetic biology. This could be achieved by in vitro reconstitution of a minimal self-sustaining central dogma consisting of DNA replication, transcription and translation. Here, we present an in vitro translation system, which enables self-encoded replication and expression of large DNA genomes under well-defined, cell-free conditions.
View Article and Find Full Text PDFPurpose: The primary tissue-site origin in over 4% of cancers remains uncertain despite thorough clinicopathological evaluation. This study assessed the effect of a Food and Drug Administration-cleared 2,000- gene-expression-profiling (GEP) test on primary tissue-site working diagnoses and management for metastatic and poorly differentiated cancers.
Methods: Clinical information was collected from physicians ordering the GEP test for patients with difficult to diagnose cancers.