Use of small fragment restriction endonuclease analysis (SF-REA) for epidemiological fingerprinting of Klebsiella oxytoca.

A group of infections caused by Klebsiella oxytoca was observed among preterm neonates in a neonatal intensive care unit (NICU) of a pediatric hospital in Osnabrück, Germany. The presence of unique antimicrobial susceptibility patterns among the bacterial isolates prompted an investigation to determine whether a limited spread of one single strain existed. All 4 K.

Genotyping of Staphylococcus epidermidis by small-fragment restriction endonuclease analysis and pulsed-field gel electrophoresis of genomic restriction fragments.

Small-fragment restriction endonuclease analysis (SF-REA) was established as a typing tool for Staphylococcus epidermidis. A total of 60 isolates comprising 48 epidemiologically nonrelated strains and 12 putatively linked isolates from 7 patients in 2 wards were analyzed. Nonrelated isolates were characterized by unique fingerprints when DNA was cleaved with EcoRI or ClaI, electrophoretically separated in a polyacrylamide gel, and silver stained.

Molecular typing of Enterobacter cloacae by pulsed-field gel electrophoresis of genomic restriction fragments.

Eleven multiply resistant Enterobacter cloacae isolates were obtained from eight preterm neonates in a neonatal intensive care unit (NICU) of one hospital in Osnabrück, together with one sensitive strain from another infant. The presence of similar antibiograms and biotypes in 11 isolates prompted further characterization of the isolates by pulsed-field gel electrophoresis (PFGE) of NotI generated genomic restriction fragments. For assessment of the discriminatory power of this typing method 50 non-related strains were included in the study.

Epidemiological fingerprinting of Enterobacter cloacae by small-fragment restriction endonuclease analysis and pulsed-field gel electrophoresis of genomic restriction fragments.

A cluster of infections caused by Enterobacter cloacae was observed among preterm neonates in a neonatal intensive care unit (NICU) of a pediatric hospital in Osnabrück, Germany. The presence of similar antimicrobial susceptibility patterns among the bacterial isolates prompted an investigation to determine whether a limited spread of a single strain existed. All 12 E.

Subtyping of Legionella pneumophila serogroup 1 isolates by small-fragment restriction endonuclease analysis.

Whole cell DNA of Legionella pneumophila isolates was examined by small-fragment restriction endonuclease analysis (SF-REA). Fourteen serogroup 1 isolates from tap water in one hospital collected before and after eradication measures had been taken were compared with control strains of serogroup 1 and other serogroups that were not epidemiologically linked. DNA was digested with EcoRI and electrophoresed on polyacrylamide gels.