Contact fluorescence imaging of reentry in monolayers of cultured neonatal rat ventricular myocytes.

Introduction: We present a novel contact fluorescence imaging (CFI) approach to monitor transmembrane potentials in monolayers of cultured neonatal rat ventricular cells. We apply CFI to demonstrate, for the first time, long-term recordings as well as electrical induction and termination of reentrant activity in this in vitro model.

Methods And Results: CFI was performed in confluent cell monolayers stained with di-8-ANEPPS.