Kinetic studies on the expression of alginate and extracellular proteins by Pseudomonas aeruginosa FRD1 and PAO1.

Pseudomonas aeruginosa is characterized by its capability to produce extracellular virulence proteins and to establish biofilm-based infections that do not respond easily to conventional treatments. However, the physiological conditions that decrease the fitness of such a persistent pathogen would assist the host to defend itself and reduce the infection prevalence. Therefore, developing treatments against P.

Expression of extracellular polysaccharides and proteins by clinical isolates of Pseudomonas aeruginosa in response to environmental conditions.

The opportunistic pathogen Pseudomonas aeruginosa causes chronic respiratory infections in patients with cystic fibrosis (CF). Persistence of this bacterium is attributed to its ability to form biofilms which rely on an extracellular polymeric substance matrix. Extracellular polysaccharides (EPS) and secreted proteins are key matrix components of P.

Technical note: A comparison of alkali treatment methods to improve neutral detergent fiber digestibility of corn stover.

The objective of this study was to compare neutral detergent fiber (NDF) digestibility of corn stover that had been treated by 2 alkali treatment methods. Two experiments were conducted to test a sodium hydroxide (NaOH) treatment method that uses an ethanol/water co-solvent (NaOH/ethanol-HO, United States Patent No. 20140220228) and a calcium hydroxide (CaOH) treatment method, which uses water as a solvent (CaOH/HO).

Irreversible transformations of native celluloses, upon exposure to elevated temperatures.

Current research, basic and applied, assumes that observed recalcitrance of celluloses is an inherent characteristic associated with their state of aggregation in their native state; it is thought that processes of isolation remove other components of plant cell walls leaving the celluloses unchanged, even though elevated temperatures are routinely used during isolation. Since temperature elevation is known to influence the structures of all polymers, it is important to explore its influence on the character of isolated celluloses, almost always assumed to be still in their native state. Deuterium exchange is a measure of accessibility of reactive sites in celluloses.