Publications by authors named "R Giovannoni"
Article Synopsis
- Checkpoint immunotherapy struggles against non-immunogenic tumors like neuroblastoma due to low MHC class I expression and neoantigen burden, but inhibiting ERAP1 can enhance anti-tumor immune responses.
- A novel strategy combining genetic knockout of ERAP1 with the HDAC inhibitor entinostat was developed, leading to increased immunogenicity and making neuroblastoma more responsive to PD-1 therapy.
- Experimental methods included CRISPR/Cas9 gene editing, flow cytometry, and mass spectrometry to demonstrate that ERAP1 inhibition improves immune cell activity and increases MHC class I and PD-L1 expression in neuroblastoma cells, ultimately slowing tumor growth.
Bioactive compounds in fruit and vegetables have a positive impact on human health by reducing oxidative stress, inflammation, and the risk of chronic diseases such as cancer, cardiovascular (CV) diseases, and metabolic disorders. However, some fruit and vegetables must be heated before consumption and thermal processes can modify the amount of nutraceuticals, that is, polyphenols, carotenoids, glucosinolates, and ascorbic acid, that can increase or decrease in relation to different factors such as type of processing, temperature, and time but also the plant part (e.g.
View Article and Find Full Text PDFis a species of great interest for numerous industrial applications. A high-quality reference genome is already available for . However, the worldwide maintenance of numerous captive populations of , each with its own genotypic and phenotypic characteristics, made it of interest to perform a de novo genome assembly on one population of to define a chromosome-scale genome assembly.
View Article and Find Full Text PDFArticle Synopsis
- - The article discusses advancements in using the CRISPR/Cas9 gene-editing system, specifically focusing on modifying a single-nucleotide polymorphism in human thyroid cells (Nthy-Ori).
- - Two delivery methods (lentivirus and chemical lipids) and two strategies for creating double-strand breaks (DSB) were tested, one using standard Cas9 and another with a modified version that creates single-strand breaks (SSB).
- - The findings suggest that using the double nickase approach is more effective for inserting genes in these cells, while the regular CRISPR/Cas9 system is better suited for knocking out genes by causing mutations.