Self-renewal, maturation, and differentiation of the rat myelomonocytic hematopoietic stem cell.

Hematopoiesis is viewed as a differentiating system emanating from a pluripotent hematopoietic stem cell capable of both self-renewal and differentiation. By identifying and characterizing a novel and highly specific in vitro mitogenic response to the N-acetyl glucosamyl/sialic acid specific, stem cell-binding lectin wheat germ agglutinin (WGA), we demonstrate the existance of a rare (0.1%), plastic adherent precursor in rat bone marrow capable of proliferation (two to seven divisions) in response to WGA.

Chromosome typing in lymphangioleiomyomatosis of the lung with and without tuberous sclerosis.

Two cases of lymphangioleiomyomatosis (LLM) of the lungs are compared, one with tuberous sclerosis and the other representing an isolated lung involvement. An increased frequency of complex chromosomal rearrangements was found in peripheral lymphocytes of the patient with tuberous sclerosis, in comparison to the patient with isolated lymphangioleiomyomatosis. Telomeric associations were found in cultured pulmonary smooth muscle cells from the patient with isolated LLM.

Chromosome abnormalities in bovine papillomavirus type 1-transformed Syrian hamster cells before and after tumor formation.

Syrian hamster embryonic fibroblasts transformed by infection with bovine papillomavirus type 1 cause tumors when inoculated into hamsters. Chromosome examinations revealed several abnormal clones in the transformed fibroblasts and a variety of additional markers in three tumors. Only one aberration, trisomy 11, was present in each cell.

Clonal chromosome aberrations in a case of cutaneous T-cell lymphoma.

Cytogenetic analysis was performed on peripheral blood and on a skin infiltration from a patient with cutaneous T-cell lymphoma. The blood cells revealed no chromosome abnormalities; whereas, besides normal cells, the skin lesion yielded on aberrant clone with the following abnormalities: trisomies 8 and 17, a deletion of the short arm of chromosome 11, and formation of one marker chromosome with possible involvement of chromosome #14.

Chromosomal changes in bovine papillomavirus type 1-induced Syrian hamster tumors.

Bovine papillomavirus type 1 (BPV-1) induced fibrosarcomas in the Syrian hamster were studied cytogenetically by G- and C-banding techniques. All tumor derived cells showed chromosome abnormalities that remained stable during serial tumor transplantations. Cells without chromosome abnormalities found in two cultures were derived from the host animals on account of heterochromatin polymorphisms.