Publications by authors named "R Gagne"

Endogenous retroviruses (ERV) are indicators of vertebrate evolutionary history and play important roles as homeostatic regulators. ERV long terminal repeat (LTR) elements may act as cis-activating promoters or trans-activating enhancer elements modifying gene transcription distant from LTR insertion sites. We previously documented that endogenous feline leukemia virus (FeLV)-LTR copy number variation in individual cats tracks inversely with susceptibility to virulent FeLV disease.

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Article Synopsis
  • Mass mortality events in wildlife can indicate new infectious diseases; in 2021, numerous dead songbirds were reported in the eastern US, showing various health issues.
  • Diagnostic tests like high-throughput metagenomic sequencing were used to analyze samples, revealing many potentially harmful microbes, mainly bacteria, but no single pathogen was consistently found among the affected birds.
  • The consistent results prompted researchers to explore other causes, such as environmental factors and nutritional issues, showcasing the value of metagenomic techniques in studying wildlife diseases and guiding future investigations.
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SARS-CoV-2 (SARS2) infection of a novel permissive host species can result in rapid viral evolution. Data suggest that felids are highly susceptible to SARS2 infection, and species-specific adaptation following human-to-felid transmission may occur. We employed experimental infection and analysis of publicly available SARS2 sequences to observe variant emergence and selection in domestic cats.

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Feline leukemia virus (FeLV) is a gammaretrovirus with horizontally transmitted and endogenous forms. Domestic cats are the primary reservoir species, but FeLV outbreaks in endangered Florida panthers and Iberian lynxes have resulted in mortalities. To assess prevalence and interspecific/intraspecific transmission, we conducted an extensive survey and phylogenetic analysis of FeLV infection in free-ranging pumas ( = 641) and bobcats ( = 212) and shelter domestic cats ( = 304).

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