Identification of a component separated on Mono Q purification of Escherichia coli RNA polymerase as an NTPase.

Standard preparations of Escherichia coli RNA polymerase (RNAP) contain NTPase activity. High-performance anion-exchange chromatography on Mono Q has recently been used by Hager et al. [1990, Biochemistry 29, 7890-7894] to fractionate RNAP into holoenzyme (alpha 2 beta beta' sigma) and core (alpha 2 beta beta') forms, plus other protein components.