In vitro growth and differentiation of primary follicles isolated from cryopreserved sheep ovarian tissue.

Achieving full in vitro growth of oocytes of both domestic animals and humans remains a major challenge. The objective of this study was to examine the in vitro development of primary follicles isolated enzymatically from cryopreserved sheep ovarian tissue. In Experiment 1, isolated primary follicles (mean diameter 60.

In vitro growth of oocytes from primordial follicles isolated from frozen-thawed lamb ovaries.

A study was conducted to develop an in vitro culture system for growing sheep oocytes from isolated primordial follicles. Enzymatically isolated neonatal sheep primordial follicles were cultured in Waymouth MB752/1 medium containing BSA (3 mg/ml) + ITS (1%, v/v) over 28 days. In Experiment 1, primordial follicles (average diameter 40.

Raman spectroscopy and imaging of beta-carotene in live corpus luteum cells.

Raman spectroscopy has been used to identify and locate beta-carotene within individual living luteal cells. The cells were either freshly prepared or cultured; the latter was incubated in the presence or absence of beta-carotene in the form of enriched bovine high-density lipoprotein. Luteal cells were investigated using several Raman spectroscopic and imaging techniques.

Effect of cyclodextrin-encapsulated beta-carotene on progesterone production by bovine luteal cells.

Experiments were conducted to examine the effect of cyclodextrin-encapsulated beta-carotene on basal or cholesterol (cyclodextrin-encapsulated), LH and dibutyryl cyclic AMP (dbcAMP)-stimulated progesterone production by bovine corpus luteum cells isolated from mid-luteal heifer ovaries by collagenase digestion. Cells were cultured with serum-free DMEM/Ham's F12 medium in serum pre-treated plastic culture dishes for periods of up to 11 days. Medium was replaced after 24h and thereafter every 48 h.

The effect of a zinc, cobalt and selenium soluble glass bolus on trace element status and semen quality of ram lambs.

Supplemental zinc and selenium were administered to ram lambs grazed on pastures that were not considered to be deficient in either element. The breeding season and polygamy of the ram mean that his requirements for semen production will be relatively large over a short breeding season and this may induce a localised deficiency of zinc and/or selenium, thus resulting in a decrease in semen quality and production.Thirty-three 8-month-old ram lambs were kept at grass and fed a supplement of barley and peas, with ad libitum access to grass silage when grazing became restricted.