Optimizing the Construction and Activation of 3D-Printed Electrochemical Sensors: An Experimental Design Approach for Simultaneous Electroanalysis of Paracetamol and Caffeine.

This work presents an optimization of the construction, treatment, and activation of 3D-printed electrochemical sensors (E-3D). For this, was used a 2-full factorial design examining three key variables at two levels: electrode height, electrode diameter, and printing speed. Moreover, it evaluates various physical, chemical, and electrochemical methods to treat and activate the E-3D surface.

Discovery of a Potent Triazole-Based Reversible Targeted Covalent Inhibitor of Cruzipain.

Cruzipain (CZP) is an essential cysteine protease of , the etiological agent of Chagas disease, and a promising druggable target. To date, no CZP inhibitors have reached clinical use, with research efforts mostly hampered by insufficient potency, limited target selectivity or lack of bioactivity translation from the isolated enzyme to the parasite in cellular environments. In this study, we report the design of , a 1,2,3-triazole-based targeted covalent inhibitor with nanomolar potency (IC = 28 nM) and null inhibition of human cathepsin L.

A transcription factor network regulates proliferation of glomerular endothelial cells in diabetic kidney disease.

The maintenance of a healthy epithelial-endothelial juxtaposition requires cross-talk within glomerular cellular niches. We sought to understand the spatially-anchored regulation and transition of endothelial and mesangial cells from health to injury in DKD. From 74 human kidney samples, an integrated multi-omics approach was leveraged to identify cellular niches, cell-cell communication, cell injury trajectories, and regulatory transcription factor (TF) networks in glomerular capillary endothelial (EC-GC) and mesangial cells.

Dental appointments in the primary health care setting for users with toothache: a longitudinal analysis.

This study aimed to estimate the morbidity associated with toothache treated in the primary health care (PHC) setting, stratified by the population size of Brazilian municipalities, from 2018 to 2022. This longitudinal ecological study was based on public national data from routine public dental service utilization records within the Health Information System for Primary Care. These data were employed to compute the rate of dental appointments for users with toothache (per 1,000 users) within each municipality throughout 15 four-month periods (P1-2018 to P3-2022), classified by population size.

Growth Differentiation Factor 15 as a Biomarker for Risk Stratification in the Cardiothoracic Surgery Intensive Care Unit.

Growth Differentiation Factor 15 (GDF15) is an emerging biomarker that significantly increases during acute stress responses, such as infections, and is moderately elevated in chronic and inflammation-driven conditions. While evidence suggests that high levels of GDF15 in cardiac surgery are associated with worse outcomes, its utility as an evaluator of early postoperative complications remains unclear. This study aims to characterize the postoperative profile of GDF15 in patients undergoing isolated surgical aortic valve replacement, evaluating its association with short-term outcomes.