Determination of amino acids in fodders and raw materials using capillary zone electrophoresis.

Two schemes were offered for analysis of amino acid contents in fodders and raw materials for mixed fodders by capillary zone electrophoresis (CZE). The first variant provides express analysis of four technologically important amino acids (lysine, methionine, threonine, cystine) in borate buffer on characteristic absorption of aminogroup (190 nm), with limits of quantitation being on average 0.2%.

[Substantiation of hygienic standard of lincomycin in the air of the work area].

The main parameters of lincomycin toxicometry were studied in animals. It was shown that the compound was low toxic after its oral or intraperitoneal administration in single doses, had no local irritant and skin resorptive effects and did not accumulate. The allergenic properties were slightly pronounced.

[Effect of sisomicin on intestinal microflora and colonization resistance in rats and their offspring].

In experiments with Wistar female rats it was shown that after administration in the form of inhalations (10.87 mg/m3) sisomicin was present in the animal feces in a concentration of 26.6 micrograms/g.

[Changes in the microbial ecology and morphology of large intestine biofilm in albino rats under the influence of rifampicin].

After oral administration in single doses of 40 and 160 mg/kg to Wistar rats rifampicin was detected in 3 hours in the contents of the large intestine The maximum rifampicin concentrations in the feces (120 and 300 micrograms/g) were detected in 24 hours. The antibiotic was present in the animal feces for 6-7 days. The antibiotic administration led to marked changes in microbiocenosis of the large intestine: disappearance of coccal flora, lower quantities of Escherichia and Lactobacillus and lower total quantities of anaerobic bacteria.

[Evaluation of the antimicrobial action of substances for the setting of hygienic standards].

The notion of antimicrobial effect threshold as an index of hygienic importance for toxicological estimation of chemotherapeutic agents is grounded by the generally accepted conception of thresholds of substances harmful effect. The methodical means used in determining the antimicrobial effect threshold including the recommended set of the measurable indices of intestinal microflora are discussed.