Publications by authors named "R F Kuo"
The copy number of a plasmid is linked to its functionality, yet there have been few attempts to optimize higher-copy-number mutants for use across diverse origins of replication in different hosts. We use a high-throughput growth-coupled selection assay and a directed evolution approach to rapidly identify origin of replication mutations that influence copy number and screen for mutants that improve Agrobacterium-mediated transformation (AMT) efficiency. By introducing these mutations into binary vectors within the plasmid backbone used for AMT, we observe improved transient transformation of Nicotiana benthamiana in four diverse tested origins (pVS1, RK2, pSa and BBR1).
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- - Magnetic nanoparticles (MNPs) are tiny particles (1 to 100 nanometers) made from magnetic materials, possessing unique properties that differ from larger forms; they are increasingly used in various fields such as medicine and technology.
- - Their small size and magnetic behavior allow for manipulation with external magnetic fields, making them useful for targeted medical applications like drug delivery and imaging, while also being explored for environmental and energy-related uses.
- - Despite the growing applications of MNPs, there are important concerns about their safety, such as potential toxicity and how they interact with cells, which is becoming a focus of both research and clinical studies.
Article Synopsis
- This study investigates the effects of Traditional Chinese Medicine (TCM) on dementia patients by analyzing real-world data focusing on clinical outcomes and medical expenses over two years.
- The research used propensity score matching and generalized estimating equations to ensure comparability and account for various influencing factors.
- Results showed that TCM significantly slowed cognitive decline in patients but also led to increased medical expenses, suggesting a need for strategic healthcare planning regarding TCM's implementation.
Development of modular expression across phylogenetically distinct diazotrophs.
J Ind Microbiol Biotechnol
January 2024
Unlabelled: Diazotrophic bacteria can reduce atmospheric nitrogen into ammonia enabling bioavailability of the essential element. Many diazotrophs closely associate with plant roots increasing nitrogen availability, acting as plant growth promoters. These associations have the potential to reduce the need for costly synthetic fertilizers if they could be engineered for agricultural applications.
View Article and Find Full Text PDFAstragalus polysaccharides (APSs), the compounds extracted from the common herb Astragalus membranaceus, have been extensively studied for their antitumor properties. In this study, we investigated the effect of APS on lung adenocarcinoma A549 cells. The effects of APS and the anti-diabetic drug metformin on apoptosis and ferroptosis were compared.
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