The Frequency of Lymphocytes Containing Dumbbell-Shaped Nuclei Depends on Ionizing Radiation Dose and Correlates with Appearance of Chromosomal Aberrations.

Nuclear anomalies of different types appear in cells in response to the action of ionizing radiation after the passage of the first mitotic division. In this article, we present the results of the study of the frequency of occurrence of three types of nuclear anomalies ("tailed" nuclei, nucleoplasmic bridges, and dumbbell-shaped nuclei) in human lymphocytes cultured with cytochalasin B when exposed to X-rays at doses of 0.0, 0.

[On the issue of non-mutagenic non-targeted effects in low renewable tissues. Analysis of low dose radiation effects on the rat renal tubule epithelium].

Irradiation of rats with γ-quanta at relatively low doses induces a sustainable dose-independent increase in the occurrence of lethal cytoplasmic disorders in the renal tubules epithelium together with sustainable and as well dose-independent subcelluar compensation and restorative processes. Over the period of research (6 months) these processes led to no population recovery. The detected alterations are referred to the category of non-targeted non-mutagenic effects and they are of interest because they address the issue of the sensitivity of low renewable tissues to radiation.

[Features of rat cells karyotypic abnormalities in rat cells in their transformation in vitro].

Neoplastic transformation of cells is characterized by karyotypic abnormalities involving aneuploidy, quantitative changes, as well as multiple clonal rearrangements of the number and structure of chromosomes. It is probable that chromosomes are one of the mechanisms of cells immortalization and transformation. Despite many years of study of chromosomal rearrangements, data on primary chromosomal rearrangements in early stages of transformation is still insufficient.

[Special mechanisms for reducing life span of cells and organisms, initiated by some weak external signals].

The study presents the results of many-years research conducted using biological objects of different organization level. It demonstrates special species-nonspecific form of weak external signals negative effect to cells life expectancy reduction caused by program damage of cells populations. This effect is detected after weak radiation, radio-chemical and thermal influences.

[Characteristics of the spontaneously transformed human endothelial cell line ECV304. II. Functional responses of the ECV304 cells].

Functional responses of the spontaneously transformed human endothelial cell line ECV304 were studied in order to asses its applicability as an endothelial cell model for studying angiogenesis and signal transduction. The dependence of proliferation activity of this line on the presence of growth factor was shown. The absent serum in culture medium resulted in blocking of cells in G1-phase of a cell cycle which is not typical for tumor cell lines.