Publications by authors named "R E Peschel"
Article Synopsis
- The import of proteins into peroxisomes relies on specific proteins: PEX5, PEX13, and PEX14.
- The SH3 domain of PEX13 plays a crucial role in binding to peptide motifs found in PEX5, impacting how these proteins interact.
- Structural studies highlight that these interactions are evolutionarily conserved, and the binding dynamics of these proteins are essential for proper functioning of peroxisomal matrix import.
Article Synopsis
- Immunoprecipitation has been the traditional method for isolating native protein complexes, but it relies heavily on antibody quality and consumes these antibodies, which limits further analyses.
- An alternative method using affinity purification is introduced, utilizing human Flp-In cells that express a Protein A-tagged version of the PEX5L receptor.
- This new method allows for the effective isolation of both soluble and membrane-bound complexes containing PEX5L through an established affinity-based approach.
Peroxisomal matrix proteins are synthesized on cytosolic ribosomes and imported in a posttranslational manner. Intricate protein import machineries have evolved that catalyze the different stages of translocation. In humans, PEX5L was found to be an essential component of the peroxisomal translocon.
View Article and Find Full Text PDFLipid flippases of the P4-ATPase family are ATP-driven transporters that translocate lipids from the exoplasmic to the cytosolic leaflet of biological membranes. In the encapsulated fungal pathogen , the P4-ATPase Apt1p is an important regulator of polysaccharide secretion and pathogenesis, but its biochemical characterization is lacking. Phylogenetic analysis revealed that Apt1p belongs to the subclade of P4A-ATPases characterized by the common requirement for a β-subunit.
View Article and Find Full Text PDFBackground: Retroperitoneal lymph node dissection (RPLND) is indicated after chemotherapy in case of radiologic incomplete remission or teratomatous elements in orchiectomy specimens. Open RPLND is associated with considerable morbidity, but technical difficulty of postchemotherapy laparoscopic RPLND (L-RPLND) can be significant; therefore, literature concerning pc L-RPLND is sparse.
Objective: To evaluate feasibility and long-term oncologic outcome of postchemotherapy L-RPLND for clinical stage II disease at a single institution.