Publications by authors named "R E Lecuona"

The boll weevil (Anthonomus grandis) is the main pest of cotton in the Americas. The aim of this work was to evaluate isolates of the entomopathogenic fungi Beauveria bassiana sensu lato and Metarhizium anisopliae sensu lato virulent against A. grandis.

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Article Synopsis
  • The study assessed cetyl trimethyl ammonium bromide (CTAB) as a new method for isolating beneficial entomopathogenic fungi compared to the existing method using dodine.
  • Oatmeal agar with chloramphenicol was utilized, testing three concentrations of CTAB, with 0.6 g/L proving most effective for promoting growth in targeted fungi while preventing non-target species from sporulating.
  • Results indicate that OA + 0.6 g/L CTAB is a cost-effective, efficient alternative for isolating specific fungi like Beauveria bassiana, Metarhizium anisopliae, and Paecilomyces lilacinus from soil samples.
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Previously undiscovered isolates of Beauveria bassiana (Balsamo) Vuillemin (Ascomycetes: Clavicipitaceae) able to control Rhipicephalus (Boophilus) microplus (Canestrini) (Acari: Ixodidae) were obtained for the first time in Argentina. The isolates were selected from three sources: 1) soil samples from the provinces of Corrientes, Formosa, and Chaco, where ticks are endemic; 2) dead female ticks; and 3) the fungal collection from the Entomopathogenic Fungi Laboratory of IMYZA-INTA Castelar. To select the isolates, population parameters were estimated, LC50 values of the most virulent isolates were calculated, and fungi-acaricides compatibility assays carried out.

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The parasitoid Spalangia endius Walker is an efficient controller of Dipteran pupae, such as Musca domestica L. The entomopathogenic fungus Beauveria bassiana (Bals.) Vuil.

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Nineteen strains and species of entomopathogenic fungi were examined for their potential as biological control agents of the house fly, Musca domestica L. Bioassays against larvae, pupae, and adult males and females in small containers indicated five strains of Beauveria bassiana (Bals.) Vuill.

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