The metabolism and transplacental transfer of oseltamivir in the ex vivo human model.

Unlabelled: Oseltamivir phosphate is extensively metabolized in the ex vivo human placenta model, and the transplacental passage of the metabolite oseltamivir carboxylate is incomplete.

Objective: To evaluate the metabolism and transplacental transfer of oseltamivir (Tamiflu) in the ex vivo human placental model.

Study Design: Perfusion studies were performed in six placentas from term, uncomplicated deliveries.

Unilateral twin ectopic pregnancy in a patient with a history of multiple sexually transmitted infections.

Background: The incidence of unilateral twin ectopic pregnancy is a rare condition. Several factors increase the risk of ectopic pregnancy, the most important of which is pelvic inflammatory disease, followed by operative trauma, congenital anomalies, tumors, and adhesions resulting in anatomically distorted fallopian tubes. We present a case of a woman with a history of four confirmed sexually transmitted infections (STIs) including Chlamydia trachomatis, Neisseria gonorrhoeae, herpes simplex virus 2, and Treponema pallidum.

Divergent activities of an engineered antibody in murine and human systems have implications for therapeutic antibodies.

The MHC class I-related receptor, neonatal Fc receptor (FcRn), plays a central role in regulating the transport and in vivo persistence of immunoglobulin G (IgG). IgG-FcRn interactions can be targeted for engineering to modulate the in vivo longevity and transport of an antibody, and this has implications for the successful application of therapeutic IgGs. Although mice are widely used to preclinically test antibodies, human and mouse FcRn have significant differences in binding specificity.

Placental transfer of rosiglitazone in the ex vivo human perfusion model.

Objective: The objective of the study was to determine transplacental passage of rosiglitazone (Avandia) using the ex vivo human placental model.

Study Design: Perfusion studies were performed on 10 placentas from term, uncomplicated deliveries. Concentrations typical for an 8-mg oral dose (216 to 692 ng/mL) as well as 2- to 3-fold increased concentrations were tested (734 to 1261 ng/mL).

Transfer of meropenem in the ex vivo human placenta perfusion model.

Objectives: To determine maternal-fetal transplacental passage of meropenem in the ex vivo human perfusion model.

Study Design: Term placentae (n = 6) were collected immediately after delivery. A single cotyledon was localized, perfused and stabilized with physiologic Eagles minimal essential medium containing 3% bovine albumin and heparin as described by Chalier (Chalier JC.