Publications by authors named "R Duplantier"
Article Synopsis
- Chancroid, a genital ulcer disease causing painful skin ulcers, is linked to a bacterium that uses a specific receptor, HgbA, to acquire heme from hosts, crucial for its disease progression.
- The study involved creating a mutant strain (35000HPΔΔ) that showed growth issues when using human hemoglobin for iron; however, trials with human volunteers showed both the mutant and the parent strain produced similar skin reactions, indicating that the YfeABCD transporter isn't essential for the bacteria's virulence.
- The research suggests that despite the deletion of the YfeABCD system, the bacteria can rely on alternative transport systems like HbpA and others to manage heme transport, highlighting the redundancy of vir
Article Synopsis
- A pathogen causes skin ulcers in tropical children and chancroid in sexually active adults, utilizing anaerobic respiration and mixed acid fermentation to generate energy in these environments.
- The study investigated the role of specific genes related to formate production and transport in a specific strain of bacteria during human infection, creating single and double mutants to explore their effects on growth and virulence.
- Results showed that while formate production is present, the lack of certain genes did not significantly affect the ability of the bacteria to cause disease, indicating that these genes are not essential for virulence in humans.
Objectives: To introduce a creatinine biosensor and a total carbon dioxide content (TCO2) method for whole-blood measurements, to evaluate the clinical performance of a new transportable analyzer that simultaneously performs these two and six other tests (Na+, K+, Cl-, glucose, urea nitrogen, and hematocrit), and to assess the potential of the new analyzer for point-of-care testing in critical care by comparing results obtained by nonlaboratory personnel and by medical technologists.
Design: Multicenter sites compared whole-blood measurements with the transportable analyzer to plasma measurements from the same specimens with local reference instruments. One site compared whole-blood results produced by nonlaboratory personnel vs.
Intestinal malabsorption is a recognized cause of malnutrition in patients infected with human immunodeficiency virus. However, the relationships among human immunodeficiency virus infection, morphological changes in the intestine, and development of intestinal malabsorption are not well established. Nine patients infected with human immunodeficiency virus underwent tests of intestinal absorption and jejunal biopsies for morphometric measurements, enzyme assays, and virus detection by in situ hybridization.
View Article and Find Full Text PDFWe used the miniature pig to evaluate the effect of ethanol ingestion on the hydrolysis of pteroylpolyglutamate and on the uptake of pteroylmonoglutamate (PteGlu) by the intestinal brush border membrane, processes that are required for folate absorption. After feeding ethanol or sucrose at 60% of calories for 11 mo, the uptake of PteGlu by jejunal brush-border-membrane vesicles was similar in both groups of animals. Jejunal brush border pteroylpolyglutamate hydrolase was decreased by one-half in the ethanol-fed group.
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