The Changes in the Antibiotic Resistance of , , and in the Clinical Isolates of a Multiprofile Hospital over 6 Years (2017-2022).

The growing resistance of bacteria to antibiotics is a serious problem in health care. The present study aims to assess the drug resistance of , , and isolated from infections in a multispecialty hospital over a 6-year period. Identification and antimicrobial susceptibility testing were performed using the VITEK2 automated system (Biomerieux).

Optimization of Cellulose Derivative-, PVA-, and PVP-Based Films with Extract to Improve Periodontal Disease Treatment.

The aim of this study was to develop and optimize polymeric films based on cellulose derivatives-hydroxypropylmethylcellulose (HPMC), methylcellulose (MC), and sodium carboxymethylcellulose (NaCMC)-as well as pullulan, polyvinyl alcohol (PVA), polyvinylpyrrolidone (PVP), and glycerol (GLY) as plasticizer incorporating extract for potential use in periodontal and gum disease treatment. Over 80 formulations were fabricated using the solvent-casting method, 6 of which were selected for further investigation based on their mechanical properties, mucoadhesion, and disintegration profiles, including three placebo films (OP1 (PVA/PVP/MC400CP/NaCMC/GLY), OP2 (PVA/PVP/MCA15C/NaCMC/GLY), and OP3 (PVA/PVP/HPMC/NaCMC/GLY)) and three films containing extract (OW1, OW2, and OW3). The films demonstrated uniform structural characteristics, with the formulations containing PVA with a high hydrolysis degree (98-99%) and methylcellulose derivatives showing prolonged dissolution times due to physical cross-linking, while the inclusion of NaCMC reduced dissolution time without compromising mucoadhesiveness.

Assessing the Interpretation of Molecular Test Results in the Diagnosis of Bloodstream Infections.

A retrospective study at the 4th Military Clinical Hospital in Wroclaw, Poland, assessed PCR testing alongside blood cultures to guide antimicrobial therapy decisions in hospitalized patients, to determine how much time the results of the molecular tests preceded conventional methods. Among 118 patients, (37%) and (21%) were the most common bloodstream infection agents. Blood cultures utilized the BacT/ALERT 3D system, and molecular diagnostics were conducted using the FilmArray platform with the BIOFIRE BCID2 panel.

Evaluation of the susceptibility of clinical isolates of NDM-producing to new antibiotics included in a treatment regimen for infections.

Background: Due to the growing resistance to routinely used antibiotics, the search for new antibiotics or their combinations with effective inhibitors against multidrug-resistant microorganisms is ongoing. In our study, we assessed the drug susceptibility of strains producing New Delhi metallo-β-lactamases (NDM) to antibiotics included in the Infectious Diseases Society of America (IDSA) and European Society of Clinical Microbiology and Infectious Diseases (ESCMID) recommendations.

Methods: A total of 60 strains of NDM-producing were obtained from different patients hospitalized at the 4th Military Hospital in Wroclaw between 2019 and 2022 and subjected to drug susceptibility to selected antibiotics, including the effects of drug combinations.

Impact of the COVID-19 Pandemic on the Epidemiology of Bloodstream Infections in Hospitalized Patients-Experience from a 4th Military Clinical Hospital in Poland.

Unlabelled: Bloodstream infections (BSIs) are associated with high mortality and inappropriate or delayed antimicrobial therapy. The purpose of this study was to investigate the impact of the COVID-19 pandemic on the epidemiology of BSIs in hospitalized patients. The research aimed to compare the incidence of BSIs and blood culture results in patients hospitalized before and during the COVID-19 pandemic.