Publications by authors named "R Diez Muino"
The success of artificial insemination (AI) with frozen-thawed semen in cattle is influenced by both female factors and sperm quality. In terms of sperm quality, prior studies indicate that the ability of frozen-thawed bovine sperm to fertilise an oocyte is dependent on their quality and resilience to cryopreservation. Cryopreservation induces oxidative stress, leading to ultrastructural damage in the sperm.
View Article and Find Full Text PDFAnogenital distance (AGD) is a marker of the degree of prenatal exposure to androgens in multiple species, and it has been suggested that there is an inverse association between AGD and fertility. Therefore, the aim of this study was to determine the usefulness of AGD and anti-Müllerian hormone (AMH) concentrations, an indirect marker of the follicular population, as predictors of future reproductive potential in Holstein cattle. The AGD was measured in 566 females from 9 dairy farms in Galicia (Spain).
View Article and Find Full Text PDFArticle Synopsis
- - The study investigates sperm cryopreservation in cattle, focusing on the types and locations of DNA damage that occur during the freezing and thawing process, particularly whether this damage includes single-strand or double-strand breaks in the DNA.
- - Results showed a significant increase in single-strand breaks post-cryopreservation, while double-strand breaks remained consistent before and after the freezing process, indicating that cryopreservation primarily affects DNA integrity through single-strand breakage.
- - The research also found that DNA fragments shortened after cryopreservation predominantly come from regions linked to protamines, suggesting cryopreservation negatively impacts these specific parts of the sperm DNA, which is crucial for successful artificial insemination.
Article Synopsis
- * Blood samples were collected at various stages around calving to assess levels of macrominerals and trace elements, and body condition scoring (BCS) was used to evaluate the health of the cows.
- * Findings indicate that optimal levels of certain minerals, particularly potassium (K) and chlorine (Cl), correlate with better reproductive efficiency, including shorter calving intervals and fewer days open, while significant changes in body condition impact reproductive outcomes.