Publications by authors named "R D Beger"
Introduction: In 2015, the FDA released a Drug Safety Communication regarding a possible link between opioid exposure during early pregnancy and an increased risk of fetal neural tube defects (NTDs). At the time, the indications for opioid use during pregnancy were not changed due to incomplete maternal toxicity data and limitations in human and animal studies. To assess these knowledge gaps, largescale animal studies are ongoing; however, state-of-the-art technologies have emerged as promising tools to assess otherwise non-standard endpoints.
View Article and Find Full Text PDFIntroduction: Coronavirus disease 2019 (COVID-19) has widely varying clinical severity. Currently, no single marker or panel of markers is considered standard of care for prediction of COVID-19 disease progression. The goal of this study is to gain mechanistic insights at the molecular level and to discover predictive biomarkers of severity of infection and outcomes among COVID-19 patients.
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- Different analytical methods in metabolomics and lipidomics include untargeted, targeted, and semi-targeted approaches, with Ultra High Performance Liquid Chromatography-Mass Spectrometry being a key tool due to its efficiency in detecting metabolites.
- The review aims to clarify the differences among these methods in terms of determining metabolite quantities and to discuss their respective advantages and limitations related to accuracy and precision.
- The choice of method is influenced by factors such as prior knowledge of metabolites, the need for peak responses or absolute concentrations, and the desired number of metabolites to analyze, with each method providing different reporting capabilities.
Article Synopsis
- Stable isotopic labeling and mass spectrometry are used in quantitative proteomics to aid in disease research, drug discovery, and biomarker identification.
- The trypsin-catalyzed O/O labeling technique is highlighted for its low sample consumption, simple process, and cost-effectiveness, making it advantageous for proteomic studies.
- The chapter outlines a protocol using this method to identify proteins related to liver damage from acetaminophen in rats, involving protein extraction, digestion, labeling, and advanced analysis of peptide fractions.
Article Synopsis
- Black cohosh extract is marketed as a dietary supplement to help relieve menopause symptoms and may protect against bone loss in postmenopausal women; however, its interactions with bisphosphonates like risedronate are unclear.
- A study on female rats examined the effects of black cohosh, risedronate, and their combination on bone mineral density over 24 weeks after ovariectomy.
- Results showed that while high doses of risedronate significantly increased bone mineral density, black cohosh extract had no significant effects alone or in combination with risedronate.