[Immunofluorescence with the PI-3 virus--study of various working conditions for the detection of viral infection using immunofluorescence on various types of cell cultures].

We used the fluorescence method for the investigation of the sensitivity of several kinds of cell cultures to the infection with the parainfluenza virus 3 (PI-3). Cultures from calf kidneys were the most sensitive while we did not determine any differences between primary cultures and cultures of the first and second subpassages and/or freshly cultivated or incubated cultures over seven days at a temperature of 37 degrees C. Equal values of infection titres like on the cultures of calf kidneys were determined by immunofluorescence also on the kidney cells of lambs though the presence of the infection was not accompanied by cytopathic changes.

[Immunofluorescence with PI-3 virus--preparation and evaluation of conjugates from immune sera of various animal species].

Immune sera with a high content of antibodies to para-influenza-3 (PI-3) virus suitable for production of specific conjugated immunoglobulins were prepared on conventionally reared calves, rabbits, and guinea-pigs, applying antigen in different ways. When processing the sera of the above mentioned animal species to conjugates, the sera differed in pure IgG yield, in completed conjugate, and molar F/P ratios, but their ability to demonstrate antigens of the PI-3 virus in the dilution was approximately the same. The serum prepared on a colostrum deprived calf showed the lowest antibody content and the lowest pure IgG yield, the colouring ability of the conjugate prepared being as well somewhat lower.

[Micro-epidemiology and micro-plaque titration of infectious bovine rhinotracheitis virus on primary calf kidney cells by means of immunofluorescence].

Immunofluorescence was used to study the virus of infectious bovine rhinotracheitis and the course of infection in cell cultures of calf kidney. An indirect relationship was found to exist between the magnitude of inoculation and the onset of specific fluorescence. Fluorescent plaques are formed as a result of inoculate dilution.

[Suitability of immune serums from different animal species for the preparation of conjugates against Aujeszky's disease virus].

A comparison was made of immune serum from rabbits, sheeps, pigs and cattle. Pure IgG was prepared by the Rivanol sulphate method and immune electrophoresis. Optimum protein quality, conjugation time under various conditions and using two preparations of fluorescein isothiocyanate were studied.