Human Papillomavirus (HPV) Detection in Cytologic Specimens: Similarities and Differences of Available Methodology.

Accumulating evidence regarding the causative role of human papillomavirus (HPV) in a wide range of malignant and nonmalignant diseases highlights the importance of HPV testing. This study describes and discusses the efficacy and characteristics of 4 well-established and commercially available tests. Here, 181 cytologic specimens from cervical smears were analyzed using the HPV SIGN PQ (Diatech) and the Linear Array (Roche) method.

Morphologic and GATA1 sequencing analysis of hematopoiesis in fetuses with trisomy 21.

Trisomy 21 alters fetal liver hematopoiesis and, in combination with somatic globin transcription factor 1 (GATA1) mutations, leads to development of transient myeloproliferative disease in newborns. However, little is known about the morphological hematopoietic changes caused by trisomy 21 in the fetus, and to date, the exact onset of GATA1 mutations remains uncertain. Therefore, we analyzed fetal liver hematopoiesis from second trimester pregnancies in trisomy 21 and screened for GATA1 mutations.

[Enzyme histochemical diagnosis of gastrointestinal motility disorders. A laboratory guide].

The enzyme histochemical reactions for acetylcholinesterase, lactic dehydrogenase, succinic dehydrogenase and nitroxide synthase are currently the gold standards for the diagnosis of gastrointestinal motility disorders. The acetylcholinesterase staining reaction shows the cholinergic nerve fibre network of the muscularis mucosae and muscularis propria, and correlates with their acetylcholinesterase activity. Lactic dehydrogenase, succinic dehydrogenase and nitroxide synthase selectively demonstrate the nerve cells of the myenteric and submucous plexus.

MIB-1 (Ki-67) immunostaining of breast cancer cells in cytologic smears.

Objective: The reliability of immunocytochemical evaluation of proliferation activity was tested using the monoclonal antibody MIB-1 on cytologic specimens.

Study Design: The study comprised 83 frozen tissue smears (FTSs) and 51 fine needle aspirates (FNAs) from 119 breast cancer patients. MIB-1 labeling indexes (LIs) were compared with various tumor parameters assessed on histologic material.