Incorporation of regulatory DNA elements within a viral vector improves recombinant protein expression in plants.

Plants have significant potential as recombinant protein expression chassis, as they can produce complex post-translationally modified proteins that are unobtainable using prokaryotic production systems, with almost limitless scalability and substantially reduced costs relative to eukaryotic cell cultures. Transient protein expression reduces the time taken between transformation and recombinant protein extraction and purification, however low protein yields relative to conventional stable expression systems remain a major obstacle. Here, we have assessed the effectiveness of combining several established genetic components, including a promoter, 5' UTR, 3' UTR, double terminator, and matrix attachment region, to modify the TMV-based pJL-TRBO expression vector for improved recombinant protein expression in plants.

Improved potency of Clofazimine derivatives against species.

Globally, millions of infections that are resistant to antimicrobial agents are reported annually, leading to more than 700,000 fatalities. Among all, challenges arise particularly from nontuberculosis mycobacterial (NTM) and Gram-negative bacteria, as they exhibit limited treatment options in light of increasing reports of multi-drug resistant strains. Clofazimine (CFZ) is an antimycobacterial medication used to treat leprosy, and it is also known for its side effect of inducing skin pigmentation.

The UK VIRTUS helmet: User feedback from Operation TORAL in Afghanistan.

In 2015, the VIRTUS helmet was introduced to UK Armed Forces and will ultimately replace the Mark 7 combat helmet. The VIRTUS helmet has a reduced trimline compared to the Mark 7 helmet and can incorporate attachments such as a visor, mandible guard and nape protection. An anonymous questionnaire was provided to 200 UK Armed Forces personnel deployed to four locations on Operation TORAL in Afghanistan between September and October 2019.

Health economic evaluations of preventative care for perinatal anxiety and associated disorders: a rapid review.

Objectives: Perinatal mental health problems affect one in five women and cost the UK £8.1 billion for every year of births, with 72% of this cost due to the long-term impact on the child. We conducted a rapid review of health economic evaluations of preventative care for perinatal anxiety and associated disorders.