Publications by authors named "R C van Waardenburg"
Article Synopsis
- Cytosolic sulfotransferases (SULTs) are important enzymes that help process both natural and foreign compounds in the body, making them easier to eliminate.
- SULT4A1, a specific type of SULT, has two mRNA variants and is primarily found in the brain, although it currently has no known enzymatic activity.
- Research suggests that SULT4A1 may play roles in neuronal development, protecting mitochondria from stress, and influencing brain functions, with potential links to conditions like schizophrenia.
Efforts to develop targetable molecular bases for drug resistance for pancreatic ductal adenocarcinoma (PDAC) have been equivocally successful. Using RNA-seq and ingenuity pathway analysis we identified that the superpathway of cholesterol biosynthesis is upregulated in gemcitabine resistant (gemR) tumors using a unique PDAC PDX model with resistance to gemcitabine acquired in vivo. Analysis of additional in vitro and in vivo gemR PDAC models showed that HMG-CoA synthase 2 (HMGCS2), an enzyme involved in cholesterol biosynthesis and rate limiting in ketogenesis, is overexpressed in these models.
View Article and Find Full Text PDFis a genetically tractable, affordable, and extensively documented eukaryotic single-cell model organism. This budding yeast is amenable for the development of genetic and biochemical experiments and is frequently used to investigate the function, activity, and mechanism of mammalian proteins. However, yeast contains a cell wall that hinders select assays including organelle isolation.
View Article and Find Full Text PDFArticle Synopsis
- The conjugation of steroids and sterols with sulfonate groups is crucial for regulating their activity, synthesis, and excretion, primarily performed by three human sulfotransferases.* -
- SULT1E1 has a high affinity for estrogen, while SULT2A1 is key for synthesizing DHEA sulfate in the adrenal gland, and SULT2B1b focuses on cholesterol sulfation and 3β-hydroxysteroids.* -
- Two effective assays are described for measuring sulfotransferase activity: one using radiolabeled substrates with organic solvent extraction and another involving PAPS and thin layer chromatography for product resolution.*